AOAC SPDS ERP DECEMBER 15, 2017

5. Based on the supporting information, what are the pros/strengths of the method?

The method did a good job with an investigation of the appropriate analytical conditions to meet the SMPR requirements.

The analytes can be determined from a single preparation and analysis.

The method provides options for the utilization of different detectors (QMS, MS/MS) to improve positive identification.

6. Based on the supporting information, what are the cons/weaknesses of the method?

No demonstration of the extraction efficiency of a single solvent preparation. They did show that the prep appears to remove all the analytes of interest with one or two extractions but there is not indication that the extraction removed all of the compound from the sample matrix. Could some percentage be bound and require a different solvent/procedure to more thoroughly extract the compounds of interest? There is also no discussion of possible extract cleanup (aside from filtration) prior to analysis. Given the potential for non-target compounds to be extracted in this procedure some thought should be given to a potential cleanup proceure. Overall it seems like it just meets the requirements of the SMPR. I would like to see additional data supporting the extraction efficiency, inclusion of matrix spiked samples, and completion of the repeatability data (Table 9).

7. Any general comments about the method?

As the method did not include data for CBCA and THCVA these compounds should not reportable under this method.

Recommendation for the Method

V. Final Recommendation Do you recommend this method be adopted as a First Action and published in the Official Methods of Analysis of AOAC INTERNATIONAL? Please specify rationale.

I do not recommend that this method be adopted at this time. I would like to see the inclusion of some type of sample spiking to demonstrate the analytical & preparation efficiency. The supporting data appears to provide some support that the procedure will work but is incomplete for some species varieties.