AOAC_SPDS_ERP_-_Set_3
AOAC SPDS ERP - Set 3 Review Form 2
Submission Date
2016-07-30 13:08:23
Name
Kurt Young
kurt.young@nutramfg.com
Organization
GNC/Nutra Manufacturing
Title of Method
Determination of Theanine in Tea
AOAC Candidate Method Number (e.g. ALN-01)
TEA-01
Applicable SMPR
2015.14
Summary:
This method utilizes ionic exchange HPLC and post-column derivitization with ninhydrin to determine the non-proteinogenic amino acid, theanine in tea, tea extracts and tea supplements. The method utilizes a lithium citrate buffer solution as a cation- exchange mobile phase component and as a sample extractant. Nor-leucine is used as an internal standard. Post-column reaction with ninhydrin at 130 deg. C is followed by UV-Vis detection at 570nm It supports the applicability of the theanine analysis portion of the SMPR. Theanine is the only amino acid constituent on the list of analytes for the SMPR, thus necessitating a separate analytical method.
1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s).
Yes, it meets all of the criteria
Yes, they are appropriately used.
The method contains a section detailing the safety and other precautions regarding reagent and instrumentation use.
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