AOAC_SPDS_ERP_-_Set_3

AOAC SPDS ERP - Set 3 Review Form 2

Submission Date

2016-07-28 12:47:46

Name

Martha Jennens

E-mail

martha.jennens@covance.com

Organization

Covance

Title of Method

Determination of Catechins and Caffeine in Camillia sinensis Raw Materials, Extracts and Dietary Supplements by HPLC-UV: Single Laboratory Validation

AOAC Candidate Method Number (e.g. ALN-01)

TEA-02

Applicable SMPR

2015.014

Summary:

A rapid method to quantify seven catechins and caffeine in green tea raw material and powdered extract and dietary supplements containing green tea extract. The method utilizes RP HPLC with a phenyl column and gradient elution with UV detection and a 13 minute run time.

1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s).

No. The method only applies to seven of eight catechins, one of three methyl xanthines (caffeine) and no theaflavin or theanine is covered.

Yes, but it does not cover all analytes in the SMPR.

Yes

There is no discussion in the method around safety/precautions in use of chemicals, etc.