AOAC SPDS Meeting Book March 19 2015

Background (Kratom Alkaloid Breakdown)

Alkaloid %

Analyte

Alkaloid %

Analyte

Mitragynine Paynantheine Speciogynine 7‐Hydroxymitragynine Speciociliatine Mitragynine oxindole B Mitrafoline Isomitrafoline Ciliaphylline

Isomitraphylline Mitraphylline Mitraciliatine Isospeciofoline Ajmalicine (Raubasine) Akuammigine Epicatechin Isopteropodine 9 Hydroxycorynantheidine ‐ Mitraversine Speciophylline Stipulatine Tetrahydroalstonine Speciofoline

66

<1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 <1

8.6 ‐ 9 6.6 ‐ 7 2 0.8 ‐ 1

<1 <1 <1 <1 <1 <1 <1 <1 <1

Corynantheidine (δ‐yohimbine) Corynoxeine Corynoxine Rhynchophylline Isorhynchophylline

Chelsea College Pharmacognosy Research Laboratories (1961-1970)

Background (Structures)

C 23 O 4 MW 398.495 H 30 N 2

C 23 O 5 MW 414.49 H 30 N 2

Mitragynine CAS no. 4098‐40‐2

7‐Hydroxymitragynine

CAS no. 174418‐82‐7