AOAC SPDS Meeting Book March 19 2015

Methods (Published)

• GC – MS • SFC Method SFC‐DAD • UHPLC MS DAD ‐ ‐ • Triple Quadrupole MS • Linear Ion Trap MS • LC‐MS with quadrupole mass spec (scan mode)

Methods (FDA)

 FDA Method

– FDA has not used a screening method – NPA submitted FOIA for FDA’s quantitative method • GC‐MS method single quadrapole – full scan spectra • Mitragynine Q Ion 214, Confirmation Ions 397, 383, 186

Scan 1705 (14.642 min): 07071404.D 397 214

800000 900000 1000000 1100000 1200000 1300000 100000 200000 300000 400000 500000 600000 700000

Spectra and Q/Confirmation Ions of 20 ug/mL mitragynine solvent standard

269

186

367

75

158

342

129

240 311

103

429 503 477 553 614641 579

50 100 150 200 250 300 350 400 450 500 550 600 0

m/z-->