AOAC SPDS Mid Year 2016

Commercial Collagen Products

1. Gelatin : • an irreversibly denatured (Heat or Acid) form of collagen (usually types I & III) used in food and cosmetics industry

2. Partially denatured (physical breakdown) or non-hydrolyzed : • All types (I/III and II are most common) • High molecular weight • Limited water solubility (soluble in mildly acidic solutions)

3 Hydrolyzed . • Type I/III (derived from beef, pig or fish skin and bones) • Type II (usually from chicken sternal cartilage), can be

SMPR Key Points

Applicability :  The method will be able to identify  and quantify individual native (un‐ denatured) and hydrolyzed collagen type I, II & III if one or multiple types  are present  in dietary ingredients  and dietary supplement finished  products.  

Validation Guidance :   Data demonstrating that a candidate  method is able to:  Separate a  bi i f i ll com nat on o nat ve co agen type I,  II and III and/or hydrolyzed collagen  type I, II and III.  Quantify each  individual collagen type both native  and hydrolyzed.  

Parameter Criteria Table 1: Method performance requirements Analytical Range (%) 1 – 100 LOQ  (%) 0.5 Recovery (%) 90‐110 % RSD r ≤ 5 % RSD R ≤ 10