AOAC SPDS Set 2 ERP

None of the alkaloids were quantitated using this method. The scope of this method may be able to be expanded to include quantitation because the reference standards were well characterized nd confirmed using TLC, HPLC, IR, 1D-NMR, 2D-NMR, and ESI-High Res-MS. The chromatographic and MS/MS conditions appear capable to allow expansion of the method’s scope to include quantitation of the analytes. Supporting Data · General Comment N/A Method Optimization Method was optimized to qualitatively assess plant products and extracts for the presence of relevant alkaloids. No quantitation of these alkaloids were presented.

· Performance Characteristics Analytical Range: N/A LOQ: N/A Accuracy/Recovery: N/A Precision (RSD r ): N/A Reproducibility (RSD R ): N/A

System Suitibility: N/A Recommendation: Do not recommend adopting this method as an AOAC OMA First Action. The method does not meet the SMPR requirements on quiantitating the relevant alkaloids. Reviewer: John Szpylka Date: 2 December 2015