AOAC SPDS Set 2 ERP

Method Review Form

AOAC INTERNATIONAL Standards Development

Name of Reviewer: Yan-Hong Wang Title of Method: LC/MS Method for the Identification of Mitragyna speciose (Kratom) and Quantitation of Mitragynine Using Linear Ion Trap Mass Spectrometer

AOAC Candidate Method Number: KRA-04

Applicable SMPR : I.

SUMMARY OF METHOD

An LC/MS method is developed for quantitative analysis of mitragynine and 7-hydroxymitragynine. The method has been applied to liquid drinks, liquid tinctures, powders, bulk ground processed leaves, dried leaves and capsules. REVIEW OF THE METHOD ONLY: 1. Does the applicability of the method support the applicability of the SMPR? If no, please explain what is missing.

II.

The method isn't validated for the parameters precision. The linear range of calibration curve is not specified.

2. Does the analytical technique(s) used in the method meet the SMPR? If no, please specify what how it differs from what is stated in the SMPR.

Yes.