AOAC SPIFAN ERP Meeting Book-March 16, 2016

VitK-01 w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Silliker Chem, Res. Center Crete, IL – Validation of a LC-MS/MS Method for Vitamin K Analysis

trans-vit K1

0.00 100000.00 200000.00 300000.00 400000.00 500000.00 600000.00 700000.00 800000.00 900000.00 1000000.00

y = 1864.4x - 6526.4 R² = 0.9994

0

100

200

300

400

500

600

Figure 2c: One of the example of the Calibration curve (linear regression) for trans-vitamin K1, Trans- vitamin K1 conc (ng/mL) (X axis) and peak area for respective concentration of the analyte (YAxis) and their linear regression relationship.

Vit K2-7

300000

y = 518.93x - 2630.9 R² = 0.9991

250000

200000

150000

100000

50000

0

0

100

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-50000

Figure 3: One of the example of Calibration curve (linear regression) for vitamin K2-7. Vitamin K2-7 conc (ng/mL) (X axis) and peak area for respective concentration(YAxis) and their linear regression relationship.

Silliker Laboratories, Chemistry Research Center, Date: 1/30/15

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