AOAC SPIFAN ERP Meeting Book-March 16, 2016

VitK-01 w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Silliker Chem, Res. Center Crete, IL – Validation of a LC-MS/MS Method for Vitamin K Analysis

Vit K2-4

100000 150000 200000 250000 300000 350000 400000 450000

y = 854.75x - 2476.9 R² = 0.9995

0 50000

0

100

200

300

400

500

600

Figure 4: One of the example of Calibration curve (linear regression) for vitamin K2-4. Vitamin K2-4 conc (ng/mL) (X axis) and peak area for respective concentration (YAxis) and their linear regression relationship. Selected calibration parameters for the targeted vitamin K compounds based on one of the calibration curve are summarized in Table 3. Table 3: Evaluation of Response Linearity against concentration of vitamin K compounds

Vitamin K1

Trans-vitamin K1

Vitamin K2-7

Vitamin K2-4

2234

1864

519

855

Slope

0.9995

0.9994

0.9991

0.9995

R^2

The calibration values based on the presented example calibration curve ( conc. Vs peak area) for different vitamin K compounds are listed in the above Table 3. All of the analytes provided a good linear response against their respective concentrations.

The calibration of vitamin K1 against ratio of peak area of vit K1 and internal std. is provided in Figure 5.

Silliker Laboratories, Chemistry Research Center, Date: 1/30/15

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