AOAC SPIFAN ERP Meeting Book-March 16, 2016

VitK-01 w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Silliker Chem, Res. Center Crete, IL – Validation of a LC-MS/MS Method for Vitamin K Analysis

Vit K1 (with ratio of int std)

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y = 0.161x - 0.133 R² = 0.9996

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Figure 5: One of the example of Calibration curve (linear regression) for vitamin K1. Vitamin K1 conc (ng/mL) (X axis) and ratio of peak area for respective concentration/peak area of internal std. (YAxis) and their linear regression relationship. The relationship between the Ratio of vitamin K1 peak area and internal std for respective vitamin K1 concentration was found to be linear , r2 = 0.9996.

Method Validation Results:

The Evaluation of Accuracy and Precision of the Method by Analysis of Reference Materials : The method accuracy was evaluated by analysis of a Reference material, NIST SRM 1849a (a milk based infant/adult nutritional formula) with a Certified value for vitamin K1 of 1.06 mcg/g + 0.17 . The NIST SRM was analyzed on five different days. The Results are presented in Table 4.

Silliker Laboratories, Chemistry Research Center, Date: 1/30/15

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