AOAC SPIFAN ERP Meeting Book-March 16, 2016

VitK-01 w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Silliker Chem, Res. Center Crete, IL – Validation of a LC-MS/MS Method for Vitamin K Analysis

Table 5 : Reconstitution of the powder samples (for evaluation of the method repeatability/precision/spike recovery evaluation) before the analysis

Powder for Reconstitution (g)

Water Added (g)

Sample #

Sample Name

Lot#ID

Child Formula Powder Infant Elemental Powder

00411RF00 00402RF00 00415RF00 00407RF00

1* 2*

12.00093 12.0037

100 100

Adult Nutritional RTF, High Protein Adult Nutritional RTF, High Fat

3*

4*

SPIFAN Blank Milk Form

Infant Formula RTF, Milk Based

5*

SRM 1849a

CLC10-b

6

12.0364

100

Adult Nutritional Powder Milk Protein Based Infant Formula Powder Partially Hydrolyzed Milk based Infant Formula Powder Partially Hydrolyzed Soy Based Adult Nutritional Powder Low Fat

11750017V3

7

12.0203

100

1172572116

8

12.0076

100

117257651Z

9

12.0867

100

00394RF00 00412RF00 00403RF00

10 11 12 13 14

12.0461 12.0862 12.00076 12.0036 12.0504

100 100 100 100 100

Child Formula Powder Infant Elemental Powder

Infant Formula Powder Milk Based Infant Formula Powder Soy Based

D04HTCVV09

E29JVL05

SPIFAN Control Milk Form

Infant Formula RTF Milk Based

15

Adult Nutritional RTF High Protein Adult Nutritional RTF High Fat

00414RF00 00406RF00

16 17

* Samples used for spike recovery evaluation.

Averages and Precision of within day Replicates analysis of SPIFAN sample tested for separate three days: Beverages prepared from the respective powdered samples and the RTF samples (as is) were analyzed for all the targeted vitamin K compounds. The Vitamin K compounds in each powder sample was analyzed in the reconstituted liquid sample. The Results of the averages and repeatability/precision (%RSD r ) for each of the sample for every day of the analysis are calculated. The results are presented in the following Table 5 a-c.

Silliker Laboratories, Chemistry Research Center, Date: 1/30/15

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