AOAC SPIFAN ERP Meeting Book-March 16, 2016

VitK-01 w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Silliker Chem, Res. Center Crete, IL – Validation of a LC-MS/MS Method for Vitamin K Analysis

Table 5a: Analysis of different SPIFAN samples in replicates on

multiple days - Day 1 Results

Day 1 of Multiple Day Replicate analysis

Total (trans + cis)

Paramtrs

Trans

Cis

Sample ID

mcg/100g of RF sample as is or a powder sample reconstituted in to a liquid

Mean

10.5

1.5 0.1 6.3 1.2 0.1 6.8 1.1 0.0 3.4 1.1 0.0 3.0 3 3 3

12.0

0.2 2.0

0.3 2.4

Stdev

SPIFAN Sample #6

% RSD

3

3

n

Mean

2.4 0.1 6.0

3.6 0.2 6.2 6.8 0.6 9.5 8.5 0.1 1.0 3.0 0.2 7.2 2.7 0.1 5.3 9.0 0.3 3.2 3 3 3 3 3

Stdev

SPIFAN Sample #7

% RSD

3

n

Mean Stdev

5.7 0.6

SPIFAN Sample #8

10.9

% RSD

3

n

Mean

7.4 0.1 1.1 2.7 0.2 6.8 2.3 0.1 4.2 8.2 0.3 3.8 3 3 3

Stdev

SPIFAN Sample #9

% RSD

3

n

Mean

0.3 0.0

SPIFAN Sample #10

Stdev

12.8

% RSD

3

n

Mean

0.4 0.0

SPIFAN Sample #11

Stdev

12.9

% RSD

3

n

Mean Stdev

0.9 0.1 7.7

SPIFAN Sample #12

% RSD

3

3

3

n

Mean

5.4 0.6

1.1 0.1

6.5 0.7

SPIFAN Sample #13

Stdev

11.0

13.1

11.4

% RSD

3

3

3

n

Mean

5.6 0.1 1.0

1.0 0.0 3.1

6.6 0.0 0.3

SPIFAN Sample #14

Stdev

% RSD

2

2

2

n

Silliker Laboratories, Chemistry Research Center, Date: 1/30/15

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