AOAC SPIFAN ERP Meeting Book-March 16, 2016

VitK-01 w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Silliker Chem, Res. Center Crete, IL – Validation of a LC-MS/MS Method for Vitamin K Analysis

The powder samples were reconstituted into the liquid form and RTF samples were analyzed as is. The results expressed as mcg/100g of vitamin K1( –trans + cis-). 6. The method interday- precision and interaday-preision was evaluated for vitamin K analysis in the SPIFAN samples and was found satisfactory for most of the analyzed samples.. 7. Five SPIFAN samples (two powders and three RTF) were spiked with targeted vitamin K compounds at three different levels.. % spike recovery of vitamin K as folic acids ranged from 97 to 100%. All the spiked samples provided a spike recovery within the recovery range 90-110% stipulated by the SMPR. The spike recovery of vitamin K compounds in the SPIFAN samples was found to be satisfactory in all of the studied SPIFAN matrices. 8. The estimated LOQ of vitamin K1 analysis method for trans, cis and total vitamin K1 is 0.50 µg/100g, 0.10 µg/100g and 0.60 µg/100g respectively in the reconstituted liquid or the RTF sample. The estimated LOD of vitamin K1 analysis method for trans, cis and total vitamin K1 is 0.15 µg/100g, 0.03 µg/100g and 0.18 µg/100g respectively

Silliker Laboratories, Chemistry Research Center, Date: 1/30/15

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