AOAC SPIFAN ERP Meeting Book-March 16, 2016
2011.06 (Fol-22) w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE
Silliker Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis
Validation of A LC-MS/MS Method for Folate Analysis in i nfant Formula and Adult Nutritional Samples
Objective:
Develop and validate a LC-MS/MS method for quantitation of folate in infant formula and adult nutritional samples
Analyte Scope:
Folic acid (FA), , 5-methyl-5,6.7,8-tetrahydrofolate (5-CH3-THF), 5-formyl-5,6.7,8-tetrahydrofolate (5-CHO-THF), 5,6.7,8-tetrahydrofolate (THF), 5,10 methenyl--5,6.7,8-tetrahydrofolate (5,10-CH=CH- THF), 10-methyl-folic acid (CH3-FA), 10-formyl-folic acid (CHO-FA).
Matrix Scope:
Infant Formula Samples Adult Nutritional
SPIFAN Samples Used for the Method Validation in this study
Provided to Silliker by SPIFAN on 3/26/14 1.) Child Formula Powder Lot # 00411RF00 2.) Infant Elemental Powder Lot # 00402RF00 3.) Adult Nutritional RTF, High Protein Lot # 00415RF00 4.) Adult Nutritional RTF, High Fat Lot # 00407RF00 5.) Infant Formula RTF, Milk Based SPIFAN Blank Milk Form The above blank samples are used for the evaluation of spike recovery of folates by the method 6.) SRM 1849A Lot # CLC10-b 7.) Adult Nutritional Powder Milk Protein Based Lot # 11750017V3 8.) Infant Formula Powder Partially Hydrolyzed Milk Based
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