AOAC SPIFAN ERP Meeting Book-March 16, 2016

2011.06 (Fol-22) w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Silliker Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis

Tetrahydrofolic acid, calcium salt 6.) 10-Methylfolic acid 7.) (6R,S)-5,10-Methylene-5,6,7,8- Tetrahydrofolic acid, calcium salt 8.) 10-Formylfolic acid

Schircks Laboratories

Cat. No. 16.211

Schircks Laboratories

Cat. No. 16.226

Schircks Laboratories

Cat. No. 16.215

9.) Pteroyltri-y-L-glutamic acid Schircks Laboratories (Folic acid, triglutamate) 10.) (6R,S)-5(Formyl-13C)-5,6,7,8- Schircks Laboratories

Cat. No. 16.253

Cat. No. 9622

Tetrahydrofolic acid, lithium salt 11.) (5-15N)-Folic Acid

Schircks Laboratories

Cat. No. 96.203

12.) 13 C-Folic Acid (Internal Standard) Merck Cat. No. CH2QC 900824 13) 13 C5-labeled (6S)-5-methyltetrahydrofolic acid, calcium salt (Internal Std) ((6S)-5-CH3-H4Pte[13C5]Glu-Ca) Merck Cat No. CH2QC 900827 14.) a-amylase Sigma-Aldrich Cat. No. A6211 15.) protease from bacillus Sigma-Aldrich Cat. No. P3910 16.) Conjugase (Rat Plasma) Bioreclamation LLC Cat. No. RATPLLIHP-M 17.) Ammonium Hydroxide Fisher Cat. No. A669-500 18.) Sodium Phosphate, dibasic salt Fisher Cat. No. S-374-500 19.) Methanol, Optima LC/MS, 99.9% Fisher Cat. No. A456-4 20.) Acetic Acid Fisher Cat. No. A38S-212 21.) Sodium Hydroxide Fisher Cat. No. S318-1 22.) Ammonium Formate Sigma-Aldrich Cat. No. 17843-250G 23.) Ammonium Acetate Fisher Cat. No. AX1220-500G 24.) 2-Mercaptoethanol Fisher Cat. No. BP176-100 25.) Ascorbic Acid Fisher Cat. No. BP351-500 26.) Water, Optima Fisher Cat. No. W7-4 27.) TCEP Fisher Cat. No. 50-093-0 28.) Charcoal Fisher Cat. No. D127-500 29) Ammonium hydroxide Fisher Cat. No. A669-500 (Ammonia soln. 28.87%)

Apparatus:

1.) Shimadzu UFLC system 2.) API 3200 Mass Spectrometer 3.) Mettler Toledo pH Meter 4.) 24-well SPE Vacuum manifold 5.) Refrigerator (capable of cooling to 4 o C) 6.) Freezer (capable of cooling to -20 o C) 7.) Water Bath, with shaker 8.) Centrifuge (capable of up to 2000rpm) 9.) Microfuge (capable of up to 15000rpm)

Supplies:

1.) Disposable Glass Culture Tubes Fisher

Cat. No. 14-961-30

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