AOAC SPIFAN ERP Meeting Book-March 16, 2016

2011.06 (Fol-22) w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Silliker Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis

442.2 460.2 460.2 474.2 474.2 446.2 446.2 456.2 456.2 456.2 456.2 470.2 470.2 447.2 465.2

176.2 313.2 194.2 327.2 299.2 299.2 166.2 412.2 282.2 309.2 176.2 295.2 176.2 295.2 313.2

75 75 75 75 75 75 75 75 75 75 75 75 75 75 75

6 6 6 6 6 6 6 6 6 6 6 6 6 6 6

6 6 6 6 6 6 6 6 6 6 6 6 6 6 6

45 28 46 25 40 30 50 42 70 20 50 35 50 20 28

6 6 6 6 6 6 6 6 6 6 6 6 6 6 6

5-CH3-THF

5-CHO-THF

THF

5,10-CH=CH- THF

10-CH3-FA

10-CHO-FA

13 C 5

-FA

13 C 5

-5-CH3- THF

DP: Declustering potential; EP Entrance potential; CEP collision cell entrance potential; CE Collision cell; CXP collision cell exit potential.

The General MS parameters for API 5500 (Qtrap):

Curtain gas(nitrogen): Collision gas(nitrogen): IonSpray voltage: Ion source gas1(zero air): Ion source gas2(zero air): Temperature:

15 psi; 5 psi; 5000 V;

650 °C; 60 psi; 70 psi;

Interface heater:

on.

MS Conditions for API 3200:

Table 8: MS conditions API 3200 for detection of different transitions of the analytes in the scope Comp Ound Q1 Q3 DP EP CEP CE CXP FA 442.2 295.2 20 4.5 39 31 6 442.2 176.2 20 4.5 39 58 6.3 5-CH3-THF 460.2 313.2 35 5 23.9 29 6 460.2 194.2 35 5 23.9 46 6 5-CHO-THF 474.2 327.2 35 4.8 21 26 7 474.2 299.2 35 4.8 21 45.5 7 THF 446.2 299.2 35 4.5 23.9 28 5.2 446.2 166.2 35 4.5 23.9 52 4 10-CH3-FA 456.2 309.2 26 4.3 30 27 6 456.2 176.2 26 4.3 30 52 4 10-CHO-FA 470.2 295.2 34 10 23.9 35 5.8 470.2 176.2 34 10 23.9 52 6.2

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