AOAC SPIFAN ERP Meeting Book-March 16, 2016

2011.06 (Fol-22) w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Silliker Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis

in a sample is calculated based on calibration curve. Concentration of each of the folate compound as ng/mL in a sample is adjusted by substracting the amount of respective folate compound detected in blank (processed like sample) ran in the batch. Concentration of each of the folate compound as ng/mL in a sample is adjusted further by multiplying with corresponding recovery factor. So estimated folate vitamer concentration in the extract is then multiplied by dilution volumes (if any) and final volumes of the relevant steps (elution volume for SPE, 1 mL). Total amount folate vitamer in the extract is then adjusted for the aliquot of the sample used for extraction. The folate vitamer then calculated as mcg/100g of the sample or any other desired unit. Total folate as folic acid is calculated in the samples mcg/100g by multiplying mcg/100g of the folate compound by the following conversion factors

Multiply by to convert to FA

Example value mcg/100g

Value as FA mcg/100g

Folate Vitamers

Formula Weight

441.4

1

sum of all folate compound as folic acid FA 5-CH3-THF 5-CHO-THF 5 ,6,7,8-THF 5,10-CH=CH- THF 10-CH3-FA 10-CHO-FA Total Folate as FA

100.0

100.0

459.5 473.4 445.4 456.4 455.4 469.4

0.9606 0.9324 0.9910 0.9671 0.9693 0.9403

100.0 100.0 100.0

96.1 93.2 99.1

100.0

96.7

100.0 100.0

96.9 94.0

Sum as FA

676.1

Reference:

Modification of the AOAC 2011.06

16