AOAC SPIFAN ERP Meeting Book-March 16, 2016
2011.06 (Fol-22) w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE
Silliker Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis
Table 24: Total folate as folic analysis in BCR 485 (dried vegetable powder) and other reference materials
Average Total Folates (mcg/g)
Expected Total Folates (mcg/g)
Sample
Matrix
Stdev
%RSD
n
days
2.293 +/- 0.062
NIST 1849A
infant formulas
2.826
0.2
6.9
23
8
NIST 3233
cereal
15.1 +/- 1.2 3.15 +/- 0.28
14.642
1.1 0.3 0.1
7.3
24 25 36
8 9
BCR 485
mixed vegetables
3.05
10.3
LCS Material
cereal
-
0.678
7.4
12
Analysis of the SPIFAN Matrices:
Samples:
A kit of all of the SPIFAN samples was received from SPIFAN in March of 2014. Sample Details are provided on page 1 and reproduced here for the convenience: Provided to Silliker by SPIFAN on 3/26/14 1.) Child Formula Powder Lot # 00411RF00 2.) Infant Elemental Powder Lot # 00402RF00 3.) Adult Nutritional RTF, High Protein Lot # 00415RF00 4.) Adult Nutritional RTF, High Fat Lot # 00407RF00 5.) Infant Formula RTF, Milk Based SPIFAN Blank Milk Form The above blank samples are used for the evaluation of spike recovery of folates by the method 6.) SRM 1849A Lot # CLC10-b 7.) Adult Nutritional Powder Milk Protein Based Lot # 11750017V3
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