AOAC SPIFAN ERP Meeting Book-March 16, 2016

2011.06 (Fol-22) w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Silliker Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis

Table 25 : Reconstitution of the powder samples (for method repeatability/precision evaluation) before the analysis

REC'D AS

Sample

Matrix

Lot #/ID

Reconstitution*

In to H 2 O (mL)

Breakfast Cereal Sample

LCS

Weight

1

POWDER 25.0926

200

SRM 1849A

CLC10-b

Adult Nutritional Powder Milk Protein Based Infant Formula Powder Partially Hydrolyzed Milk Based Infant Formula Powder Partially Hydrolyzed Soy Based Adult Nutritional Powder Low Fat Infant Formula Powder Milk Based Infant Formula Powder Soy Based Infant Formula RTF Milk Based Adult Nutritional RTF High Protein Adult Nutritional RTF High Fat Child Formual Powder Infant Elemental Powder

2

POWDER 25.0158

200

11750017V3

3

POWDER 25.0324

200

1172572116

4

POWDER 25.0356

200

117257651Z

5

POWDER 25.0142

200

00394RF00

6

POWDER 25.0264

200

00412RF00

7

POWDER 25.0108

200

00403RF00

8

POWDER 25.017

200

D04HTCVV

9

POWDER 25.1178

200

E29JVLV

SPIFAN CONTROL MILK FORM

10

LIQUID

-

-

11

LIQUID

-

-

00414RF00

12

LIQUID

-

-

00406RF00

* All the samples received as powder were recostituted and and the reconstituted beverage was analyzed for folates.

Averages and Precision of within day Replicates analysis of SPIFAN sample tested for separate three days: Nine beverages prepared from the respective powdered samples and three RTF samples were analyzed for all the targeted folate compounds. The folate compounds in each powder sample was calculated on as is basis (powder basis). Similarly the folate compounds in the RTF samples were also calculated as is basis (liquid basis). Each folate compound analyzed was converted into folic acid and sum of that provided total folate contents in the sample (expressed as folic acid) and reported as mcg/g (ppm). Each sample was analyzed in three replicates for three separate days. Results for the averages and repeatability/precision (%RSD r ) for each for every day of the analysis are calculated. The results are presented in the following Table.

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