AOAC SPIFAN ERP Meeting Book-March 16, 2016

FOS-01 w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

The fructans are extracted from the sample with boiling water. An aliquot of the extract is hydrolyzed. The concentrations of glucose, fructose and sucrose are chromatographically determined. The description of the GC-method can be found in annex 4.

2.3Validation

2.3.1 Precision. All samples of the Test Materials kit were screened for the presence of fructans. Four samples were found to contain amounts above the LOQ limit of 0.03 g/100 g reconstituted product.

Adult nutr powder Milk Protein based

11750017V3 00729RF00 00411RF00 00412RF00

J4G-00019 J4G-00980 J4G-00013 J4G-00974

Adult nutr RTF high Fat Child formula powder Child formula powder

These samples were analyzed in duplicate, on 6 different days and by different operators. As the method is consisting of different steps, the hydrolysis, silylation, integration were performed by different people. The RSDr and RSD(ir) were calculated from the obtained data. 2.1.2 Accuracy. For the accuracy, a spiking was done by the 3 different raw materials provided by the Test Materials kit.

Nutraflora P95 (FOS)

J4G-00010

Orafti P95 (FOS)

J4G-00011

Orafti HP (inulin)

J4G-00012

The Nutraflora and Orafti P95 are short chain fructo-oligosaccharides with DP ranges from 2 to 8. Nutraflora is produced from sugar as raw material while Orafti P95 is originating from chicory roots. The Orafti HP is a long chain inulin extracted from chicory with DP ranges from 2 until 60.

p.4