AOAC SPIFAN ERP Meeting Book-March 16, 2016

FOS-01 w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

4. Conclusion.

The method used for this SLV meets the requirements as described in the AOAC SMPR 2014.002 for fructans in Infant Formula and Adult/pediatric Nutritional Formula in which fructan is present in a concentration ranging from 0.03 to 0.5 g/100g reconstituted final product. The precision as done during the SLV meets the repeatability as proposed by the workgroup, being ≤6%. The accuracy was performed by spiking the Test Materials kit samples by 3 different pure fructan products of different origin (sugar and chicory) and DP range. The spiking/recovery is ranging from 96.9 to 107.1% with a mean of 100.3%, which is very well meeting the proposed requirement of 90-110%. The very low concentrations (spike level 0.05) are included during this spiking test and even these meet the proposed range.

Annex 1 Chromatogram of a standard. Annex 2 Calibration curve for fructose and sucrose. Annex 3 Chromatogram of a sample J4G0975 (Infant elemental powder 00403RF00). Annex 4 Description of the GC method.

Tiense suikerraffindarij/ Beneo-Orafti (part of Südzucker group) Central Department Research, Development and Services-Analytics-Tienen Monique Steegmans

p.8