AOAC SPIFAN Nutrients ERP Report (July 28, 2022)
Method Title
Reviewer(s)
Vote
Method FLUORIDE
Recommendations
(Fluor-06) - HS-GC analysis of fluoride in infant formula and adult nutritionals
Motion: Method was not recommended for First Action Official Methods SM status Tom Vennard motioned Brendon Gill second No action taken
See ERP recommendations:
Tom Vennard Brendon Gill
FLUOR-06 RECOMMENDATIONS FROM ERP: 1. Revise method with calibration standard preparation, system suitability, quality control procedures, and safety statement 2. Validation of reconstitution procedure 3. Clarify table 1 for analytical range and LOQ 4. Add information on method specificity and the impact of acid treatment on bound fluorine 5. Describe how innate fluoride concentrations were obtained for the spiked experiments 6. Explain the calibration curves use for each spiking level 7. Add chromatograms of mid-level calibration, matrix w/out fluoride spike and matrix with fluoride spike 8. Experimental data which focuses on the appropriateness of using TMCS in an aqueous environment 9. If possible, compare method to the Willard-Winter method Motion to approve recommendations: Tom Vennard motioned & Brendon Gill second
Unanimous
Vote
Method
Method Title
Comments
Reviewer(s)
Motion: Method was not recommended for First Action Official Methods SM status Brendon Gill motioned Hans Cruijsen second Esther Campos Gimenez abstains
PHOSPHOLIPIDS
(Phospho-02) - Quantification of phospholipids by UPLC MS/MS
Brendon Gill Hans Cruijsen/ Martine van Gool
See ERP recommendations:
PHOSPHOLIPIDS RECOMMENDATIONS FROM ERP: 1. Concern using non-bovine sourced phospholipids a. The limited range of samples used; want to see a wider range of samples of those particular standards 2. Recommend use of internal standard 3. Clarification on sample intake or reconstitution for different parts of the SLV
4. Clarification on the spiking and calculations 5. Clarification on accuracy of PS against PNMR a. Clarification on comparison result outside 80-120% 6. Include safety statement 7. Explore alternatives to the use of chloroform
Motion: Brendon Gill & Hans Cruijsen Abstain: Nestle
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