AOAC SPIFAN ERP Reviewer Forms (March 16, 2017)

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Expert Review Panel for Infant Formula and Adult Nutrition

Evaluation of Method _MTE-01/MTE-03

Title: AOAC 2011.14 ISO/CD 15151 | IDF 229 Infant formula, Adult nutrition Milk and milk products — Determination of calcium, copper, iron, magnesium, manganese, phosphorus, potassium, sodium and zinc contents – Inductive coupled plasma atomic emission spectrometric method (ICP-AES) (January 2017)

Author: Hans Cruijsen, FrieslandCampina, The Netherlands Eric Poitevin, Nestle, Switzerland

Summary of Method: The organic matter is decomposed by wet digestion using nitric acid in a pressurized microwave assisted wet digestion system or any appropriate instrumentation in wet digestion and diluted. The test and calibration solutions are atomized into plasma of an inductively coupled plasma spectrometer and the emission is measured at appropriate wavelengths using external calibration. Method Scope/Applicability: This method is applicable to calcium, copper, iron, magnesium, manganese, phosphorus, potassium, sodium, and zinc contents in milk and milk products. The method has been tested with the SPIFAN sample set as well as formula milk, milk powder, whey powder, whey protein concentrate, butter and processed cheese. General comments about the method: 1) Method is well written. 2) Use microwave to assist sample digestion, sample preparation time is about 70 min. 3) Various ICP-AES instrument systems were used in the study. These include ICP-AES from Perkin Elmer, Thermo, Agilent, Varian, Spectro. Different models from Perkin Elmer were used also. 4) Comparing to the existing AOAC Final method that using ICP-MS, both methods has similar sample prep (microwave, nitric acid), and both tested with butter, cheese, whey powder, etc. in addition to the SPIFAN sample set. The ICP-MS method has been validated on additional three elements (chromium, selenium, and molybdenum).

Method Clarity: Method is well written, clear to follow

Method Safety Concerns: Safety hazards are addressed.

Pros/Strengths: • ICP-AES instrument from different vendors and different models from the same vendor were used in the MLT.

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