AOAC SPIFAN Nutrients ERP (December 7, 2022)

Amino-07 (SLV) 10-2022 FOR ERP USE ONLY DO NOT DISTRIBUTE

0 ′ = 0 √

eqn 14

standard deviation

where: 0 ′ = standard deviation 0 = concentration = = 3 × 0 ′ where: 0 ′ = standard deviation 0 = concentration = = × 0 ′ where: 0 ′ = standard deviation =

estimated standard deviation at or near zero

number of values in data set

eqn 15

limit of detection

estimated standard deviation at or near zero

number of values in data set

eqn 16

limit of quantitation

multiplier whose reciprocal equals the selected quantifying relative standard deviation

Bias against reference material hypothesis test 0 : = 0

eqn 17 where: 0 = hypothesised difference in measured results by method and reference value = mean difference between test methods or between test method and reference value

= − 0 �

test statistic

eqn 18

2 + � � 2

where: = test statistic; expression using sample

estimates of characteristics of the population = mean of value of data set 0 = reference value of material = standard deviation of differences between the measured results and reference value = number of replicate analyses = uncertainty of the reference value = coverage factor used for calculating the expanded uncertainty