AOAC SPIFAN Nutrients ERP (December 7, 2022)

Amino-07 (SLV) 10-2022 FOR ERP USE ONLY DO NOT DISTRIBUTE

Appendix C: Data—Linearity Table C1: Linearity experiment day 1 Taurine ( μ g mL − 1 ) 13 C 2 15 N Taurine ( μ g mL − 1 )

Peak Area Ratio

Concentration Ratio

0.06

6 6 6 6 6 6 6 6 6

0.01 0.04 0.84 0.41 0.83 4.04 7.78

0.01 0.04 0.08 0.42 0.85 4.06 8.38

0.3 0.6

3 6

30 60

300 600

35.41 65.15

34.12 66.64

Regression y = 0.6659 x + 0.4023, r 2 = 0.9993

Table C2: Linearity experiment day 2 Taurine ( μ g mL − 1 ) 13 C 2 15 N Taurine ( μ g mL − 1 )

Peak Area Ratio

Concentration Ratio

0.06

6 6 6 6 6 6 6 6 6

0.01 0.04 0.84 0.41 0.83 4.04 7.78

0.01 0.04 0.84 0.41 0.83 4.04 7.78

0.3 0.6

3 6

30 60

300 600

35.41 65.15

35.41 65.15

Regression y = 0.6581 x + 0.4527, r 2 = 0.9983