AOAC SPIFAN Nutrients ERP (December 7, 2022)

For ERP/Working Group Use Only DO NOT DISTRIBUTE Fluor-04 (SLV) Resubmission FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC SMPR 2014.016 Method Performance Requirements

Limit of Quantitation (LOQ)

30

30 – 200 b

Analytical Range

30 – 100 b 80 - 120

> 100 b 90 - 100

Recovery, %

Repeatability (RSD r ), % Reproducibility (RSD R ), %

≤ 8

≤ 5

≤ 15

≤ 10

a Concentrations apply to: (a) ‘ready-to-feed’ liquids “as-is”

(b) reconstituted powders (25 g into 200 g deionized water) (c) liquid concentrates diluted 1:1 by weight with deionized water b µg/100 g reconstituted final product

Results

Precision Fluoride concentrations in the samples were calculated using equations generated daily by the standard curves. The results were then converted to meet the SMPR units of µg fluoride / 100 g reconstituted product using the following equations: ( / ) = 10 ( ) − � � = ( / ) ∗ ℎ ( ) ℎ ( ) * ( ) ( ) Where: F = µg fluoride in g powder Dilution weight = Weight of beaker, sample, & reagents – tare weight Recon weight = weight of reconstitution tested Sample weight = weight of product used to make reconstitution Total recon weight = sample weight + water weight used to make recon The concentration of fluoride per 100-gram reconstituted final product was then calculated: � 100 � = � � ∗ 2 2 2 5 5

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