AOAC SPIFAN Nutrients ERP (December 7, 2022)

For ERP/Working Group Use Only DO NOT DISTRIBUTE Fluor-04 (SLV) Resubmission FOR ERP USE ONLY DO NOT DISTRIBUTE

The LODs calculated (2.4 for electrode #1 and 2.1 for electrode #2) agree with the manufacturer’s suggested LOD of 2.0 µg/ 100g. Likewise, the LOQs of 3.7 and 3.2 confirm the estimation of 4 µg/ 100 g. Data generated by the blank samples can be found in Table V .

Table V: Method blank data supporting LOQ calculation

Electrode # 1 (x i -MT  ) 2

Electrode # 2 (x i -Orion  ) 2

Electrode #1 (µg/100g)

Electrode # 2 (µg/100g)

Day

Rep #

1 2 1 2 1 2 1 2 1 2 1 2

1.82 1.44 2.04 1.66 1.91 1.65 1.96 1.61 2.03 1.70 1.96 1.63

0.001 0.119 0.065 0.017 0.016 0.019 0.032 0.029 0.058 0.006 0.031 0.023

1.68 1.44 1.85 1.51 1.79 1.51 1.75 1.50 1.88 1.55 1.79 1.50

0.001 0.041 0.041 0.018 0.022 0.017 0.010 0.021 0.054 0.010 0.020 0.021

1

2

3

4

5

6

1.78

1.65



SD r

0.195

0.159

2.4

2.1

LOD (  + 3 SD r )

3.7

3.2

LOQ (  + 10 SD r )

Ruggedness All matrices were tested on two electrodes from different vendors. The RSD ip of the samples - including those at or below the LOD - ranged from 0.6 – 7.7%, meeting the SMPR repeatability requirement of ≤ 8%. (Refer to Table I ). When comparing the data generated by each electrode, the ratios of the averages were 1.0 ± 0.03, indicating no bias between electrodes ( Table VI ).

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