AOAC SPIFAN Nutrients ERP (December 7, 2022)

Fluor-06 (SLV) 10-2022 FOR ERP USE ONLY DO NOT DISTRIBUTE

1 Summary

A single laboratory validation study was performed for the method “Quantitative analysis of fluoride in infant formula and adult nutrition using headspace gas chromatography with a flame ionization detector”. The performance characteristics are summarized below.

Parameter

Results

Linearity and working range Limit of quantification (LOQ) Selectivity and specificity

0.17 – 67 mg/kg powder (1.9 – 741 µg/100 g reconstituted final product) a 0.17 mg/kg powder (1.9 µg/100 g reconstituted final product) a

Retention time: 9.6 minutes Retention time (comparison): standard/sample < 2.5%

Recovery

92 — 105% 0.9 — 2.9% 1.8 — 8.0%

Repeatability Reproducibility

Robustness 0.0 — 2.4% a reconstituted powders (25 g into 200 g water)

The proposed analytical method meets all the stated method performance requirements reported by AOAC International for fluoride.