AOAC SPIFAN Nutrients ERP (December 7, 2022)

HMO-02 (Analysis Paper) 10-2022 FOR ERP USE ONLY DO NOT DISTRIBUTE

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Figure 1: Typical Chromatograms of 7 HMOs. A: 7 HMOs spiked in bovine milk, showing adequate separation between lactose phosphate and 2'FL. B: HMOs in an infant formula with partially hydrolyzed proteins. 2’FL = 2’-fucosyllactose, LacPO4 = lactose phosphate, 3FL = 3-fucosyllactose, IS = internal standard, DFL = difucosyllactose, 3’GL = 3’-galactosyllactose (naturally present in bovine milk), 6’GL = 6’-galactosylactose (naturally present in bovine milk), 3’SL = 3’-sialyllactose, LNT = lacto-N-tetraose, LNnT = lacto-N-neotetraose, 6’SL = 6’sialyllactose

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