AOAC SPIFAN Nutrients ERP (December 7, 2022)

HMO-02 (Analysis Paper) 10-2022 FOR ERP USE ONLY DO NOT DISTRIBUTE

Spike Level 1 (mg/100g) a

Spike Level 2 (mg/100g) a

Spike Level 3 (mg/100g) a

Spike Level 4 (mg/100g) a

Matrix

HMO

2’FL 3FL DFL LNT

5.09 4.03 1.58 2.01 5.19 1.50 1.94 5.09 4.03 1.58 2.02 5.19 0.00 1.48 5.09 4.03 1.60 2.06 5.41 0.00 0.00 5.08 4.02 1.83 2.16 5.18 3.20 2.28 15.2 147 2.99 7.06 10.2 1.17 3.17

20.3 28.9 9.98 19.8 21.3 21.0 22.4 20.3 28.9 10.0 19.9 21.3 21.1 22.5 20.1 28.8 10.2 20.2 21.3 21.0 22.9 20.2 28.7 19.9 21.2 21.2 25.1 24.6 21.3 98.7 3.27 11.3 20.9 2.19 8.39

98.7 98.5 45.9 119 72.2 67.3 71.8 99.6 99.4 46.3 120 72.8 67.9 72.5 98.8 98.5 46.7 121 72.2 67.3 73.4 98.7 98.4 50.1 98.4 72.1 79.1 84.4 30.7 48.6 5.18 28.1 40.8 4.05 13.5

494 587 96.3 292 101 144 148 495 591 97.1 296 104 139 144 496 584 98.7 294 101 143 144 477 600 95.6 304 106 142 147 151 16.0 21.1 50.9 77.3 14.0

Infant Formula Powder Soy-Based

LNnT 3’SL 6’SL

2’FL 3FL DFL LNT

Infant Formula Powder with Partially Hydrolysed Protein and Probiotics

LNnT 3’SL 6’SL

2’FL 3FL DFL LNT

Infant Formula Powder, Goat Milk Based

LNnT 3’SL 6’SL

2’FL 3FL DFL LNT

Infant Formula RTF with FOS & GOS

LNnT 3’SL 6’SL

2’FL 3FL DFL LNT

Infant Formula Powder with FOS & GOS [2] c

LNnT 3’SL 6’SL

19.3 a Concentrations expressed as concentration found in reconstituted powders (25 g + 200g water) or “ready-to-feed” liquid. b Matrix from the SPIFAN 2 matrices kit. c Infant formula powders with GOS and FOS [1] and [2] are two different products and contain different sources of GOS.

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