AOAC SPIFAN Nutrients ERP (December 7, 2022)

HMO-02 (Analysis Paper) 10-2022 FOR ERP USE ONLY DO NOT DISTRIBUTE

remains below the lowest limit of the desired working range of 1.5 mg/100g. The method may thus remain fit for purpose. Table 9: Estimated LoD and LoQ for each HMO in different matrices Matrix HMO Mean a (mg/100g) SD a (mg/100g) LoD a (mg/100g) LoQ a (mg/100g) Cow Milk Formula with FOS 2’FL 0.305 0.014 0.35 0.44 Formula with Partially Hydrolysed Protein 2’FL 0.306 0.013 0.34 0.43 Formula with Extensively Hydrolysed Protein 2’FL 0.253 0.021 0.32 0.46 Soy Based Formula 2’FL 0.328 0.027 0.42 0.61 Water 2’FL 0.349 0.016 0.40 0.51 Cow Milk Formula with FOS 3FL 0.253 0.011 0.28 0.36 Formula with Partially Hydrolysed Protein 3FL 0.243 0.008 0.27 0.33 Formula with Extensively Hydrolysed Protein 3FL 0.241 0.007 0.26 0.31 Soy Based Formula 3FL 0.281 0.006 0.30 0.34 Water 3FL 0.278 0.013 0.32 0.41 Cow Milk Formula with FOS DFL 0.830 0.040 0.95 1.2 b Formula with Partially Hydrolysed Protein DFL 0.585 0.020 0.65 0.79 Formula with Extensively Hydrolysed Protein DFL 0.407 0.093 0.69 1.3 b Soy Based Formula DFL 0.497 0.041 0.62 0.91 Water DFL 0.195 0.012 0.23 0.31 Cow Milk Formula with FOS LNT 0.090 0.013 0.13 0.22 Formula with Partially Hydrolysed Protein LNT 0.092 0.011 0.13 0.21 Formula with Extensively Hydrolysed Protein LNT 0.090 0.012 0.13 0.21 Soy Based Formula LNT 0.180 0.016 0.23 0.34 Water LNT 0.093 0.022 0.16 0.31 Cow Milk Formula with FOS LNnT 0.290 0.012 0.32 0.41 Formula with Partially Hydrolysed Protein LNnT 0.284 0.010 0.13 0.21 Formula with Extensively Hydrolysed Protein LNnT 0.299 0.019 0.36 0.49 Soy Based Formula LNnT 0.385 0.027 0.47 0.65 Water LNnT 0.260 0.019 0.32 0.45 Soy Based Formula 3’SL 0.089 0.010 0.12 0.19 Water 3’SL 0.092 0.013 0.13 0.22 Soy Based Formula 6’SL 0.456 0.035 0.56 0.81 Water 6’SL 0.112 0.023 0.18 0.35 a Concentrations expressed as concentration found in reconstituted powders (25 g + 200g water) or “ready-to-feed” liquid. b The estimated LoQ is at or higher than the LoQ defined in the SMPR (1.2 mg/100g for DFL).

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