AOAC SPIFAN Nutrients ERP (December 7, 2022)

HMO-02 (Analysis Paper) 10-2022 FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 10: Spike-recovery and precision estimates for 2'FL

Matrix Conc. (mg/100g)

Spike Conc. (mg/100g)

Recovery (%)

RSD rob (r) (%)

RSD rob (iR) (%)

Sample Description

n

6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 7×2 7×2 7×2 7×2 6×2 6×2 6×2 6×2 6×2 6×2

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

4.87 20.7 103 511 5.93 20.2 101 504 5.17 20.5 102 499 5.09 20.3 98.7 494 5.09 20.3 99.6 495 5.09 20.1 98.8 496 5.08 20.2 98.7 477 15.2 21.3 30.7 151

99.0 103 102 101 98.5 100 103 101 104 104 103 102 101 105 104 103 100 103 103 103 104 104 104 103 100 102 102 101 103 102 102 101

1.0 0.7 0.6 0.3 1.2 0.8 0.2 0.6 1.1 1.0 0.7 0.6 0.4 0.5 0.7 0.5 0.7 0.9 0.7 0.7 0.7 0.7 0.6 0.2 0.4 1.3 0.6 0.6 0.7 0.5 1.5 0.2 1.0 1.1 0.9 0.7 1.0 0.2 0.9

1.3 1.2 0.6 0.5 1.3 2.6 0.9 1.2 1.4 0.8 0.5 1.2 1.1 1.5 1.3 1.2 1.2 1.3 1.0 1.6 0.9 0.8 0.9 1.0 2.5 2.5 2.8 2.3 1.8 1.4 1.5 0.4 1.6 1.9 1.8 1.8 1.5 2.0 2.4

Infant formula powder with FOS & GOS [1]

Adult nutritional RTF with FOS & GOS

Infant formula elemental powder

Infant formula powder soy based

Infant formula powder with partially hydrolysed protein and probiotics

Infant formula powder goat milk based

Infant formula RTF with FOS & GOS

Infant formula powder with FOS & GOS [2]

Commercial infant formula powder with GOS & 2’FL Commercial infant formula powder with probiotic & 2’FL Commercial infant formula RTF with 2’FL Commercial infant formula powder with 2’FL & LNnT Commercial infant formula powder with 2’FL, LNnT & GOS Pilot infant formula powder with 2’FL and LNnT Pilot infant formula powder with GOS and HMOs (Lab reference sample)

80.8 19.7 24.6 86.4 22.8 86.3 153*

- - - - - - -

- - - - - - -

25×2

All results reported on a “ready-to-feed” basis except * reported as the concentration in the non-reconstituted powder

25