AOAC SPIFAN Nutrients ERP (December 7, 2022)

HMO-02 (Analysis Paper) 10-2022 FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 13: Spike-recovery and precision estimates for LNT

Matrix Conc. (mg/100g)

Spike Conc. (mg/100g)

Recovery (%)

RSD rob (r) (%)

RSD rob (iR) (%)

Sample Description

n

6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 7×2 7×2 7×2 7×2 6×2 6×2 6×2 6×2 6×2 6×2

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

2.01 20.6 147 294 2.24 20.2 147 287 1.96 21.3 148 290 2.01 19.8 119 292 2.02 19.9 120 296 2.06 20.2 121 294 2.16 21.2 98.4 304 7.06 11.3 28.1 50.9

101 106 103 101 101 106 106 105 106 104 104 107 107 107 105 99.8 107 106 104 98.1 103 104 103 106 104 102 100 106 104 102 100 113 a

0.8 0.6 0.6 0.5 4.2 1.0 0.5 0.4 1.0 0.4 0.6 0.4 1.0 0.3 0.5 0.6 1.0 0.8 0.6 0.7 1.6 0.9 0.4 0.2 2.1 1.2 0.8 0.7 1.1 0.3 0.9 0.2

2.5 1.3 0.5 1.1 3.6 2.3 0.5 1.4 2.0 1.1 1.1 1.4 1.1 1.1 1.1 1.1 1.4 1.1 0.8 1.6 1.8 0.8 0.6 1.0 9.0 5.3 3.4 1.6 3.4 3.1 2.5 1.9

Infant formula powder with FOS & GOS [1]

Adult nutritional RTF with FOS & GOS

Infant formula elemental powder

Infant formula powder soy based

Infant formula powder with partially hydrolysed protein and probiotics

Infant formula powder goat milk based

Infant formula RTF with FOS & GOS

Infant formula powder with FOS & GOS [2]

Commercial infant formula powder with GOS & 2’FL Commercial infant formula powder with probiotic & 2’FL Commercial infant formula RTF with 2’FL Commercial infant formula powder with 2’FL & LNnT Commercial infant formula powder with 2’FL, LNnT & GOS Pilot infant formula powder with 2’FL and LNnT Pilot infant formula powder with GOS and HMOs (Lab reference sample)

- - - - - - -

- - - - - - -

- - - - - -

- - - - - -

25×2

52.5*

1.4

2.8

All results reported on a “ready-to-feed” basis except * reported as the concentration in the non-reconstituted powder a Performance does not meet SMPR

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