AOAC SPIFAN Nutrients ERP (December 7, 2022)

HMO-02 (Analysis Paper) 10-2022 FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 14: Spike-recovery and precision estimates for LNnT

Matrix Conc. (mg/100g)

Spike Conc. (mg/100g)

Recovery (%)

RSD rob (r) (%)

RSD rob (iR) (%)

Sample Description

n

6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 7×2 7×2 7×2 7×2 6×2 6×2 6×2 6×2 6×2 6×2

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

6.08 23.1 73.9 103 5.56 22.2 74.8 103 5.35 23.4 75.6 103 5.19 21.3 72.2 101 5.19 21.3 72.8 104 5.41 21.3 72.2 101 5.18 21.2 72.1 106 10.2 20.9 40.8 77.3

100 102 102 101 99.1 102 104 104 111 a 106 103 104 102 105 106 104 99.9 104 105 104 102 102 106 105 103 102 105 104 104 105 106 104

0.5 0.5 0.5 0.3 0.7 1.2 0.6 0.4 0.8 0.4 1.4 0.4 0.8 0.3 0.7 0.9 1.0 0.8 1.0 0.7 0.7 1.3 0.4 0.7 1.1 1.1 0.7 0.5 0.5 0.3 0.9 0.2

0.7 1.1 1.0 1.2 5.6 3.3 1.1 1.2 1.5 1.2 2.2 1.2 2.0 1.6 1.3 1.1 1.1 1.2 1.4 2.0 1.2 1.1 0.6 1.6 3.3 3.5 2.7 1.6 2.5 1.6 2.6 2.9

Infant formula powder with FOS & GOS [1]

Adult nutritional RTF with FOS & GOS

Infant formula elemental powder

Infant formula powder soy based

Infant formula powder with partially hydrolysed protein and probiotics

Infant formula powder goat milk based

Infant formula RTF with FOS & GOS

Infant formula powder with FOS & GOS [2]

Commercial infant formula powder with GOS & 2’FL Commercial infant formula powder with probiotic & 2’FL Commercial infant formula RTF with 2’FL Commercial infant formula powder with 2’FL & LNnT Commercial infant formula powder with 2’FL, LNnT & GOS Pilot infant formula powder with 2’FL and LNnT Pilot infant formula powder with GOS and HMOs (Lab reference sample)

- - - - - - -

- - - - - - -

- - -

- - -

43.6 10.7 43.7

1.4 1.1 0.1 1.0

3.1 2.0 1.5 2.0

25×2

95.8*

All results reported on a “ready-to-feed” basis except * reported as the concentration in the non-reconstituted powder a Performance does not meet SMPR

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