AOAC SPIFAN Nutrients ERP (December 7, 2022)

HMO-02 (Analysis Paper) 10-2022 FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 16: Spike-recovery and precision estimates for 6'SL

Matrix Conc. (mg/100g)

Spike Conc. (mg/100g)

Recovery (%)

RSD rob (r) (%)

RSD rob (iR) (%)

Sample Description

n

6×2 6x2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6×2 6x2 6×2 6×2 6×2 6×2 6x2 6×2 6×2 6×2 6x2 6×2 6×2 6×2 6×2 6x2 6×2 6×2 7×2 7×2 7×2 7×2 7×2 6×2 6×2 6×2 6×2 6×2 6×2

0.82 0.82 0.82 0.82

-

-

2.6 0.9 0.4 0.3 2.9 0.5 0.7 0.5 0.9 1.0 0.6 0.5 1.4 1.0 0.7 1.3 0.4 0.2 0.7 0.4 3.8 2.0 1.2 0.6 0.7 1.9 1.5 0.3 0.5 1.2 0.7 0.5 0.7 1.8 0.4 0.7 1.4 0.1 2.4 0.7 0.9 0.6 0.7 1.5 4.1 1.2 2.7 1.6 0.7 1.0

3.4 2.1 2.9 1.9 3.4 3.7 1.9 1.8 4.5 2.6 2.3 1.8 3.9 2.2 2.7 4.2 6.9 1.4 1.7 1.7 5.5 1.4 1.8 0.6 0.9 3.8 3.1 1.9 2.6 2.8 3.1 1.1 2.7 7.9 3.9 2.9 2.4 2.0 4.6 5.2 3.5 2.7 3.5 2.7 6.4 4.2 10 6.2 2.9 3.0

21.2 78.4 148 2.23 24.4 80.2 142 1.77 24.6 78.1 142 1.77 24.6 78.1 142 1.94 22.4 71.8 148 1.48 22.5 72.5 144 22.9 73.4 144 22.9 73.4 144 2.28 24.6 84.4 147 3.17 8.39 13.5 19.3 - - - - -

106 105 102 109 105 107 107 106 105 104 103 105 102 103 108 107 102 103 106 106 104 109 106 103 103 103 103 103 105 104 102 103 103 102 101 168 a 121 a - - - - -

Infant formula powder with FOS & GOS [1]

- - - - - - - - - - - - - - - -

Adult nutritional RTF with FOS & GOS

Infant formula elemental powder

Infant formula elemental powder (75°C elution)

Infant formula powder soy based

0.56 0.56 0.56 0.56 0.56 0.83 0.83 0.83 0.83 0.90 0.90 0.90 0.90 1.15 1.15 1.15 1.15 1.15 1.35 1.31 1.16 1.35 0.83 0.72 0.52 1.31 0.39 1.31 1.09

Infant formula powder with partially hydrolysed protein and probiotics

Infant formula powder goat milk based

Infant formula powder goat milk based (75°C elution)

Infant formula RTF with FOS & GOS

Infant formula powder with FOS & GOS [2]

Commercial infant formula powder with GOS & 2’FL Commercial infant formula powder with probiotic & 2’FL Commercial infant formula RTF with 2’FL Commercial infant formula powder with 2’FL & LNnT Commercial infant formula powder with 2’FL, LNnT & GOS Pilot infant formula powder with 2’FL and LNnT Pilot infant formula powder with GOS and HMOs (Lab reference sample)

- - - - - - -

- - - - - - -

25×2

42.4*

All results reported on a “ready-to-feed” basis except * reported as the concentration in the non-reconstituted powder. a Performance does not meet SMPR

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