AOAC SPIFAN Nutrients ERP (December 7, 2022)
Amino-06 (SLV) 10-2022 FOR ERP USE ONLY DO NOT DISTRIBUTE
Table 4. Mass spectrometer instruments settings
Parameter Curtain gas Collision gas
Values 30 psi
6 psi
IonSpray Voltage
5500 V 600 °C 50 psi
Temperature
Ion Source Gas 1
Ion Source Gas 2
70 psi
Declustering Potential Entrance Potential
65 V 10 V
Collision gas
N 2
Table 5. MS component settings
MRM parameters
Q1 Mass (Da)
Q3 Mass (Da)
Component
CE (V) CXP (V) Dwell time (ms)
Taurine (Qualifier) Taurine (Quantifier)
126,1 126,1
108,0
15 27 27
10
150 150 150
44,1 48,1
8 8
2-Aminoethane-d 4 -sulfonic acid (IS) 130,1
K. System suitability
The chromatographic system should be equilibrated before starting a series of analysis. Check if the column backpressure and the signal baseline are stable. Start a series with a set of six injections with a continuing calibration verification (CCV) standard followed by a system blank and the calibration standards (T0-T6). The used solution for the CCV is calibration standard 3. The taurine peak of the CCV should elute at a retention time of 4,05±0,25 minutes with a peak asymmetry of 1,0±0,4. In the system blank no taurine or taurine-IS should be detected .
L.
Quality control
After the calibration standards start with a CCV and reinject after every ten analyzed samples. The recovery of the CCV should be between 95-105%. The ion ratio between the taurine qualifier ion and the taurine quantifier ion is determined. The ion ratio of a sample is allowed to deviate 15% from the average of the standards.
M. Calibration and calculation a. A weighted calibration line is established every analysis. The calibration line is analyzed before the analysis of samples and is based on six concentrations. A linear weighted model with offset is used. The accuracy of the line is checked every ten samples with the CCV. The instrument software is used to plot the calibration line where the correct peak area
Made with FlippingBook - Share PDF online