AOAC SPIFAN Nutrients ERP (December 7, 2022)

Amino-06 (SLV) 10-2022 FOR ERP USE ONLY DO NOT DISTRIBUTE

from the standard (area response taurine(quantifier)/area response 2-aminoethane-d 4 sulfonic acid) is plotted against the concentration of total taurine in the calibration standard with a 1/x weighted factor. The correlation coefficient (R 2 ) of the calibration line should be ≥ 0,998. The amount of taurine in an injected sample is calculated by the software with the use of corrected peak area and a weighted calibration model. b. Calculate the amount of taurine in RTF or reconstituted sample with equation 1. The amount of total taurine in the sample will be expressed in mg/100 g.

Equation 1.

× 50 × 5 × 1000 × 10

=

Where:

Tau

Taurine content in RTF or reconstituted sample in mg/100g

C Tau

Concentration of taurine in injection sample in µg /L

50

Total volume of clarified sample in mL

5

Dilution of sample

M s Mass of the test sample in grams 1000 Conversion factor from µg/L to mg/L 10

Conversion factor from mg/kg to mg/100 g

The amount of taurine expressed as mg/100g in powdered sample or liquid concentrated sample can be calculated with the help of equation 2.

Equation 2.

= ×

Where:

Tau powder

Taurine content in powdered or liquid concentrated sample in mg/100g

V r

Total weight of powder or liquid concentrated with water in grams

M r

Weight of powder or liquid concentrated sample in grams

Tau

Taurine content in liquid concentrated or reconstituted sample in mg/100g