AOAC SPIFAN Nutrients ERP (December 7, 2022)

Amino-07 (Method) 10-2022 FOR ERP USE ONLY DO NOT DISTRIBUTE

Table XXXX.XXC. Mass spectrometer instrument settings a Parameter

Value

ESI +

Ionization mode

Curtain gas

30 40 40

Nebulizer gas GS1

Heater gas GS2 Collision gas

N 2 (medium)

Source temperature Ion spray voltage

300°C

5500 V a These settings are suitable for the 6500 triple quadrupole mass spectrometer (Sciex); optimal settings on alternative instruments may differ.

(b) The specific compound parameters to be used are:

Table XXXX.XXD. Compound parameters

Precursor ion,

Product ion,

DP

b ,

EP c ,

CE d , CXP e , Dwell time,

Ion a

V

V

V

V

ms

m/z

m/z

quantifier qualifier

126.0 126.0 129.0

108.0

15 49 15

16 16 16

150 150 150

65.1

1

10

internal standard

111.0

a quantifier = taurine quantifier ion, qualifier = taurine qualifier ion, internal standard = 13 C 2

15 N taurine

ion. b DP = declustering potential. c EP = entrance potential. d CE = collision energy. e CXP = collision cell exit potential.

I.

Calculations (a) Concentration of 13 C 2 15 N taurine in stable isotope labelled stock standard ( SILSS ): SILTSS = 1 1 0 0 0 × 1000 where: SILSS Tconc = concentration of 13 C 2 15 N taurine in SILSS (μg mL −1 ) 10 = mass of 13 C 2 15 N taurine in SILSS (mg)