AOAC SPIFAN Nutrients ERP (December 7, 2022)

Amino-07 (Method) 10-2022 FOR ERP USE ONLY DO NOT DISTRIBUTE

100 = final of volume of SILSS (mL) (b) Concentration of taurine in non-labelled stock standard ( NLSS ): NLSS = 1 1 0 0 0 0 × 1000 where: NLSS Tconc = concentration of taurine in NLSS ( μ g mL − 1 ) 100 = mass of taurine in NLSS (mg) 100 = final of volume of NLSS (mL) (c) Concentration of taurine in non-labelled working standard ( NLWS ): NLWS = NLSS × 2 2 . 5 5 where: NLWS conc = concentration of taurine in NLWS ( μ g mL − 1 ) NLSS conc = concentration of taurine in NLSS ( μ g mL − 1 ) 2.5 = volume of NLSS in NLWS (mL) 25 = final of volume of NLWS (mL) (d) Concentration of taurine in calibration standards ( CS1–5 ): CS1 conc = NLWS Tconc × 0 6 .0 .3 3

CS2 conc = NLWS Tconc × 0 6 .1 .3 5 CS3 conc = NLWS Tconc × 0 6 . . 3 3 CS4 conc = NLWS Tconc × 1 6 . . 5 3 CS5 conc = NLWS Tconc × 3 6 . . 0 3 where:

CS1–5 conc = concentration of non-labelled taurine in CS1–5 ( μ g mL − 1 ) NLWS conc = concentration of non-labelled taurine in NLWS ( μ g mL − 1 ) 0.03, 0.15, 0.3, 1.5, 3.0 = volume of NLWS in CS1–5 (mL)