AOAC SPIFAN Nutrients ERP (December 7, 2022)

Amino-07 (SLV) 10-2022 FOR ERP USE ONLY DO NOT DISTRIBUTE

Table of Contents Summary................................................................................................................................. 2 Introduction ............................................................................................................................. 4 Principle .................................................................................................................................. 4 Validation ................................................................................................................................ 4 Samples .................................................................................................................................. 5 Linearity .................................................................................................................................. 5 Precision ................................................................................................................................. 8 Limit of Quantitation ................................................................................................................ 9 Accuracy ............................................................................................................................... 10 Robustness ........................................................................................................................... 13 Instrumental Analysis............................................................................................................ 15 System Suitability.................................................................................................................. 16 Quality Control ...................................................................................................................... 17 Acknowledgements............................................................................................................... 17 References............................................................................................................................ 17 Appendix A: SMPR 2014.13 ................................................................................................. 19 Appendix B: Equations.......................................................................................................... 22 Appendix C: Data—Linearity................................................................................................. 28 Appendix D: Data—Precision ............................................................................................... 30 Appendix E: Data—Limit of Quantitation .............................................................................. 37 Appendix F: Data—Bias against CRM.................................................................................. 38 Appendix G: Data—Bias against AOAC 997.05 ................................................................... 40 Appendix H: Data—Recovery ............................................................................................... 41 Appendix I: Data—Robustness............................................................................................. 44