AOAC SPIFAN Nutrients ERP (December 7, 2022)

Amino-07 (SLV) 10-2022 FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 10: Taurine recovery of spiked adult nutritional formula sample

Recovery (%)

Spiked adult nutritional formula

Standard Deviation

95% Confidence Interval

Mean

low concentration (~0.7 mg hg − 1 ) medium concentration (~7 mg hg − 1 ) high concentration (~70 mg hg − 1 )

97.2 99.2

5.0 3.9 2.5

95.6–98.9 97.9–100.6 99.3–101.0

100.1

Recovery at SMPR concentration ranges Recovery at the concentration ranges specified in SMPR 2014.013 were evaluated by spiking an adult nutritional formula at three concentration levels in the ranges: low (~2 mg hg − 1 RTF), medium (~20 mg hg − 1 RTF), and high (~200 mg hg − 1 RTF). Spiked samples, unspiked samples, and spiked standards as independent replicates eight times with average recoveries compared to the requirements specified in SMPR 2014.013 (Table 11; Appendix H).

Table 11: Taurine recovery of spiked adult nutritional formula sample

Recovery (%)

Spiked adult nutritional formula

95% Confidence interval

SMPR limit Mean Standard deviation

low concentration (~2 mg hg − 1 RTF) medium concentration (~20 mg hg − 1 RTF) high concentration (~200 mg hg − 1 RTF)

100 ± 12 100 ± 10 100 ± 7

99.2

4.6 4.3 5.9

96.1–102.2 99.5–105.2 97.6–105.3

102.3 101.4

Average method recoveries were estimated from 99.2–101.4 %, which are within the limits set in the SMPR 2014.013.

Robustness Robustness is normally expressed as the lack of influence on the test results of operational variables of the analytical method. A robustness trial is used to evaluate the effect on test results of the variation in conditions normally expected from a test method. The robustness of the method was assessed by conducting a Plackett–Burman trial [10, 11] with graphical and statistical interpretation of results [12, 13]. The seven factors were assessed in the study and evaluated whether statistically significant changes in measured results occurred due to the method changes for each factor (Table 12; Eqns 27–31; Appendix I).

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