AOAC SPIFAN Nutrients ERP-Final Review (Jan. 2019)

2017.03 (JULY 2018) Amino-02 Tryptophan by enz hyd_Abbott Labs_Method

FOR ERP USE ONLY DO NOT DISTRIBUTE

HPLC Determination of Total Tryptophan in Infant Formula and Adult/Pediatric Nutritional Formula Following Enzymatic Hydrolysis

Jonathan Draher and Norman White Abbott Nutrition, Division of Abbott Laboratories

L . CONTRIBUTING REFERENCES

1. Narahashi, Y.; Yanagita, M. : (1967) J. Biochemistry 6 pp 633-641. 2. Yamaskov, I.A.; Tichonova, T.V.; Davankov, V.A.; (1986) ENZYME MICROB. TECHNOL. 8 pp241-244. JM . SPECIFICITY - Selectivity for the analyte of interest is supported by the following: 1. The selectivity of chromatographic separation combined with native fluorescence 2. Near 100% recovery of reference material

Page

26