AOAC SPIFAN Nutrients ERP-Final Review (Oct. 2018) Pubs

2011.06 (September 2017)-Fol-22 MLT Report

FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 4-C Preparation of Folate Calibration std. solutions

Volume of 2 ppm Internal Std Soln. (2 µ g/mL) (µL)

Volume of 40 ppb Intl Std Soln. (40ng/mL) (µL)

Volume of Intermediate/ Calibration STD Soln. (uL)

Volume of Neutr Elution Solv. (µL)

Conc. of each folate compound in the std. solution ng/mL

Calibr ation STD

Final Vol (mL)

25 uL Intermed. Std. soln. 10 uL Intermed. Std. soln. 100 uL Calibration std Soln A 100 uL Calibration std Soln B 100 uL Calibration std Soln C 100 uL Calibration std Soln D 50 uL Calibration std Soln D

1.0 mL

A

500

20

955

0

20

1.0 mL

B

0

200

970

900

1.0 mL

C

50

0

0

D

20

900

1.0 mL

0

0

0

900

E

1.0 mL

5

0

900

1.0 mL

F

2

0

0

950

1.0 mL

G

1

0

0

Note: Internal std. conc in every calibration std. solution (A through G) = 40 ng/mL of each folate internal std. compound.

Collaborative Study Report: Method AOAC 2011.06 (Folate by LC-MS/MS) 33