AOAC SPIFAN Nutrients ERP-Final Review (Oct. 2018)

2011.06 (September 2017)-Fol-22 MLT Report

FOR ERP USE ONLY DO NOT DISTRIBUTE

4.) Pteroyltri-y-L-glutamic acid (Folic acid, triglutamate) Schircks Laboratories

Cat. No. 16.253

5.) 13 C 5 IsoSciences, PA Cat# 14139 (Please mention to vendor that the order is for the AOAC MLT study to receive discounted price of US $ 300 discount study price). 6.) 13 C 5 -labeled (6S)-5-methyltetrahydrofolic acid, calcium salt IsoSciences, PA Cat# 14168Ca (Please mention to vendor that the order is for the AOAC MLT study to receive discounted price of US $ 300 discount study price). 7) 13 C 5 -labeled (6S)-5-formyltetrahydrofolic acid, calcium salt, ((6S)-5-CH3-H4Pte[13C5]Glu-Ca) Merck Cie . This internal std. Chemical will be provided to each participant by the study director in about 1 mg amount. This is expensive chemical (costing more than US $ 5400). All chemicals used in preparation of standards were stored at -20°C II - Other Chemicals: Stored per manufacturer guidelines or set conventions. Listed chemicals were used in the validation study. The equivalent chemicals/enzymes could be used from alternate sources 8.) a-amylase Sigma-Aldrich Cat. No. A9857 9.) protease from Bacillus licheniformis , Subtilisin Sigma-Aldrich Cat. No. P3910 10.) Conjugase (Male Rat Plasma) with Lithium and heparin, Bioreclamation LLC Cat. No. RATPLLIHP-M, buy at least 2 of 10 mL vials. We may reserve a batch of the Rat plasma after testing its suitability for the study with the company. -Folic Acid (Internal Standard)

We shall provide the lot# for ordering separately. 11.) Ammonium Hydroxide, 28-30% w/w Fisher

Cat. No. A669-500

12.) Sodium Phosphate, dibasic salt

Fisher

Cat. No. S-374-500

13.) Methanol, Optima LC/MS, 99.9% Fisher

Cat. No. A456-4

14.) Glacial Acetic Acid

Fisher

Cat. No. A38S-212

15.) Sodium Hydroxide

Fisher

Cat. No. S318-1

16.) Ammonium Formate

Sigma-Aldrich

Cat. No. 17843-250G

17.) Ammonium Acetate

Fisher

Cat. No. AX1220-500G

18.) 2-Mercaptoethanol

Fisher

Cat. No. BP176-100

Sigma M6250

Collaborative Study Report: Method AOAC 2011.06 (Folate by LC-MS/MS) 26