AOAC SPIFAN Nutrients ERP-Final Review (Oct. 2018)

2011.06 (September 2017)-Fol-22 MLT Report

FOR ERP USE ONLY DO NOT DISTRIBUTE

Curtain gas(nitrogen):

30 psi;

Collision Assisted Dissociation

Medium;

IonSpray voltage:

5500 V;

Temperature:

400 °C;

Ion source gas1(zero air):

60 psi;

Ion source gas2(zero air):

50 psi

MS Conditions for the Sciex 6500 Plus LC-MS/MS: MS conditions Sciex 6500 for detection of different transitions of the analytes in the scope Comp ound Q1 Q3 DP EP CXP CE

442.2

295.2

75

6

6

20

FA

75

6

6

442.2

176.2

45

75

6

6

460.2

313.2

28

5-CH3-THF

75

6

6

460.2

194.2

46

75

6

6

474.2

327.2

25

5-CHO-THF

75

6

6

474.2

299.2

40

75

6

6

13 C 5

-FA

447.2

295.2

20

75

6

6

13 C 5

-5-CH3-THF

465.2

313.2

28

75

6

6

13 C 5

-5-CHO-THF

479.2

327.2

25

DP: Declustering potential; EP Entrance potential;

Collaborative Study Report: Method AOAC 2011.06 (Folate by LC-MS/MS) 42