AOAC SPIFAN Nutrients ERP Method Reviews (September 7, 2019)

Yes. There is no requirement of any specific technique in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. * Yes 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s). * Yes, the method description contains a Safety Precaution section, in which the potential safety hazards are given. 1. Are the definitions specified in the SMPR used and applied appropriately in the supporting documentation (manuscripts, method studies, etc...)? If not, please explain the differences and if the method is impacted by the difference. * Yes 2. Is there information demonstrating that the method meets the SMPR Method Performance Requirements using the Reference Materials stated in the SMPR? If not, then specify what is missing and how this impacts demonstration of performance of the method. * There is no reference materials specified in the SMPR. The SRM 1869a has been tested in the SLV report, but there is no certified value available for an evaluation of accuracy. 3. Is there information demonstrating that the method performs within the SMPR Method Performance Requirements table specifications for all analytes in the SMPR applicability statement? If not, please specify what is missing and whether or not the method's applicability should be modified. * In the SLV report, Table 1 shows the repeatability and intermediate precision for 11 SPIFAN samples, including a SRM 1869a, in the concentration range from 4.85 ug/100g to 135 ug/100g. The repeatability results meet the SMPR requirement in different analytical ranges (30-100, and above 100 ug/100g). Table 1 also shows the accuracy obtained from spiking experiment. The accuracy results are within the 90-110%. However, the spiking levels in those samples were not shown in Table 1. Table II shows the linearity over the standard range of 2-200 ug/100g. III. Review of Information in Support of the Method III. Review of Supporting Information