AOAC SPIFAN Nutrients ERP Method Reviews (September 7, 2019)

FOR EXPERT REVIEW PANEL USE ONLY NOT FOR DISTRIBUTION

It would be useful for the authors to include actual concentrations of amino acids found in SPIFAN matrices as well as actual spike levels used for spike-recoveries studies for total amino acids.

Method Clarity: Clearly written, lists all the reagents, standards and supplies

Pros/Strengths: For total amino acids • Uses established methodology and commonly available equipment

• Fast chromatographic analysis with good peak separation • Separates Citrulline from proteinogenic amino acids • Method describes all steps and procedures to prepare reagents, standards and samples • Linearity, accuracy and repeatability for SPIFAN matrices are evaluated and compared with SMPR For free amino acids • Uses established methodology and commonly available equipment • Fast chromatographic analysis with good peak separation • Separates Citrulline from proteinogenic amino acids • Method describes all steps and procedures to prepare reagents • Linearity and other parameters are evaluated using spikes of SRM 1869 and compared with SMPR Cons/Weaknesses For total amino acids Not suitable for Tryptophan For free amino acids Only spikes of SRM 1869 were used for SLV Cystine and Cysteine are not separated

Supporting Data

• Performance Characteristics:

Analytical Range: Calibration range Low level calibration standards: 0.1-0.5 mg/100 mL High calibration standards: 2-10 mg/100 mL

2