AOAC SPIFAN Nutrients ERP Method Reviews (September 7, 2019)

FOR EXPERT REVIEW PANEL USE ONLY NOT FOR DISTRIBUTION

LOQ: Calculated using standard deviation of low amino acids area response and slope of the calibration curves. All LOQ met SMPR

Accuracy/Recovery: Total amino acids analysis

Accuracy was determined by analyzing NIST SRM 1849a and 1869. Results for all amino acids were within reference range and within 97%-100% from respective mean Reference Mass Fraction Value. The only exception was result for Tyrosine in NIST SRM 1849a. The results met SMPR Accuracy was also confirmed by analyzing lactose spiked with amino acids at 3 levels. Recoveries ranged from 93-107 % meeting SMPR. The only exception were lower recoveries for Methionine. Actual spiking levels are not listed Free amino acids analysis Accuracy was evaluated by analyzing NIST SRM 1869 spiked at 3 levels. Recoveries met SMPR Precision (RSD r ): Total amino acids analysis Repeatability (RSD r ) and Intermediate Reproducibility (RSD iR ) were calculated for 17 matrices. RSD r ranged from 0.4 to 2.7, RSD iR ranged from 1.5 to 4.8. All results met SMPR Free amino acids analysis Repeatability was evaluated by analyzing SRM 1869 spiked at 3 different levels. The data for RSD r met SMPR Reproducibility (RSD R ): NA

• System suitability:

System suitability requirements and quality control procedures are listed

Recommendation:

I recommend the method for determination of total proteinogenic amino acids and taurine for First Action AOAC Official method. More SPIFAN matrices or matrices containing free amino acids should be evaluated before recommending the method for free amino acids analysis.

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