AOAC SPIFAN Nutrients ERP Method Reviews (September 7, 2019)

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Method Scope/Applicability: This method is applicable to infant and adult/pediatric nutritional formulas and other matrices such as infant cereals and pet foods.

General comments about the method: The method is very sensitive and can go down to approximately ten times lower than the SMPR requirements. The sensitivity of method is a direct reflection of its signal to noise ratio which ensures guaranteed method performance at the lower levels of amino acids in these matrices. The method offers baseline separation of citrulline which is an alpha amino acid generally present in Infant Formula and Adult / Pediatric Nutritional products. The separation of citrulline eliminates the risk of interference of this compound with other amino acids. Hydroxyproline is an important component of collagen and often this amino acid is used for quantitation of collagen .

Method Clarity: The method is clear, well written and easy to follow.

Method Safety Concerns: Not special safety concerns are needed. The analyst may be familiar with safety practices for all the chemicals and equipment utilized.

Pros/Strengths: • The method is simple and does not include any proprietary chemicals or instruments and can be performed on any basic UHPLC system. • The method incorporated an optional fluorescence detector as a precautionary measure to manage matrix interference and confirmation. • The method allows the determination of several amino acids in one single analysis. Supporting Data • General Comment : Certified Reference Materials NIST SRM 1849a and 1869 were used for the evaluation of method accuracy and precision. A sample of Whey Protein Concentrate and a sample Goat Milk based Infant Formula were also included in the validation. A total of 17 matrices were used for the single laboratory validation study.

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